P30 Prof. Dr. Kramann
Dissecting pathways initiating a profibrotic and anti-angiogenic injury response in perivascular myofibroblast precursors in kidney fibrosis
Our data indicates that injury drives detachment of Gli1+ cells from capillaries triggering renal capillary rarefaction, hypoxia and myofibroblast formation as initiators of fibrosis. In an unbiased Gli1 cell-specific transcriptomic approach we identified critical pathways involved in this process that might be promising therapeutic targets. The project will utilize cell specific in vivo CRISPR/Cas9 gene editing to induce conditional loss of function mutations in these pathways using bigenic Gli1CreERt2; Cas9 mice with the ultimate goal to stop initiation of kidney fibrosis by switching Gli1+ cells from a pro-fibrotic, anti-angiogenic towards a pro-angiogenic pro-regenerative phenotype. [...]
Publications
- Kuppe C. , Ibrahim M. M., Kranz J., Zhang X., Ziegler S., Perales-Patón J., Jansen J., Reimer K. C., Smith J. R., Dobie R., Wilson-Kanamori J. R., Halder M., Xu Y., Kabgani N., Kaesler N., Klaus M., Gernhold L., Puelles V. G., Huber T. B., Boor P., Menzel S., Hoogenboezem R. M., Bindels E. M. J., Steffens J., Floege J., Schneider R. K., Saez-Rodriguez J., Henderson N. C., Kramann R. (2020): Decoding myofibroblast origins in human kidney fibrosis. nature, 589, 281–286,
- Tajti F. , Kuppe C., Antoranz a., Ibrahim M. M., Kim H., Ceccarelli F., Holland C. H., Olauson H., Flege J., Alexopoulos L. G., Kramann R., Saez-Rodriguez J. (2019): A functional landscape of chronic kidney disease entities from public transcriptomic data. Kidney International Reports, [2.260]
- Sun Q. , Baues M., Klinkhammer B. M., Ehling J., Djudjaj S., Drude N. I., Daniel C., Amann K., Kramann R., Kim H., Saez-Rodriguez J., Weiskirchen R., Onthank D. C., Botnar R. M., Kiessling F., Floege J., Lammers T., Boor P. (2019): Elastin imaging enables non-invasive staging and treatment monitoring of kidney fibrosis. Sci Transl Med, 11, 486, [17.161]
- Saez-Rodriguez J. , Rinschen M.M., Floege J., Kramann R. (2019): Big Science and Big Data in Nephrology. Kidney International, 95(6), 1326-1337, [8.306]
- Puelles V. G. , Fleck D., Ortz L., Papadouri S., Strieder T., Boehner A., van der Wolde J. W., Vogt M., Saritas T., Kuppe C., Fuss A., Menzel S., Klinkhammer B. M., Müller-Newen G., Heymann F., Decker L., Braun F., Kretz O., Huber T. B., Susaki E. A., Ueda H. R., Boor P., Floege J., Kramann R., Kurts C., Bertram J. F., Spehr M., Nikolic-Paterson D. J., Moeller M. J. (2019): 3D analysis of rapidly progressive glomerulonephritis using optical tissue clearing. Kidney Int, 96, 505-516, [8.306]
- Yagmur, E. , Koch, A., Haumann, M., Kramann, R., Trautwein, C., Tacke, F. (2012): Hyaluronan serum concentrations are elevated in critically ill patients and associated with disease severity. Clinical Biochemistry, 45, 82-87, [2.382]
- Kramann, R. , Moeller, M. J. (2011): The next level of complexity: post-transcriptional regulation by microRNAs. Kidney International, 80 (7), 692-693, [7.683]
- Kramann, R. , Couson, S. K., Neuss, S., Kunter, U., Bovi, M., Bornemann, J., Knuchel, R., Jahnen-Dechent, W., Floege, J., Schneider, R. K. (2011): Exposure to uremic serum induces a procalcific phenotype in human mesenchymal stem cells. Arterioscler Thromb Vasc Bio, 31 (9), e45-54, [5.969]